General Cell Based Assays
Eurofins Discoverx PathHunter Human NPY1R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human NPY1R β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure NPY1R (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter eXpress CXCR1 (IL8RA) CHO-K1 GPCR Assay, 10-Plate Kit. (95-0073E2CP2L)
The cAMP Hunter CXCR1 (IL8RA) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR1, a naturally Gi/Go coupled GPCR. Upon activation of CXCR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human ADRA1D (82-572) β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human ADRA1D (82-572) β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure ADRA1D (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TrkB Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human TrkB Stable Cell Line Functional Kinase Assay (U2OS) measures TrkB receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress A549 G9a Methyltransferase Assay, 10-plate Kit. (96-0003E15CP7L)
The InCELL Hunter eXpress A549 G9a Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR7 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0528PE2MCP0M)
The PathHunter mCCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of mCCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR7 and EA-tagged β-Arrestin-2. Activation of mCCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human Axl Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human Axl Stable Cell Line Functional Kinase Assay (U2OS) measures AXL receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IL-4 (IL4R/IL2RG) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-4 (IL4R/IL2RG) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL4R/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human IL-11 (IL11RA) Signaling Reporter Cell-Based Assay Kit (HepG2)
The PathHunter Human IL-11 (IL11RA) Signaling STAT3 Reporter Stable Cell Line Assay (HepG2) measures STAT3 pathway activation through detection of induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Biolegend Alexa FluorR 647 anti-AMPKalph
Alexa Fluor 647 anti-AMPKα Phospho (Thr172) [A20017A]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICFC; Size: 100 μg
Meso Scale Discovery U-PLEX Human A2M ASY 25 PL
The Meso Scale Discovery U-PLEX Human A2M Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Alpha-2-Macroglobulin (A2M) in human serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and accurate detection of A2M levels down to sub-pg/mL concentrations The assay requires 50 L sample volume and can be read on MSD instrument platforms This product provides a simple rapid way to quantify A2M levels which can aid research into inflammatory conditions immune function and proteolytic regulation
Meso Scale Discovery R-PLEX Human IL-36 ASY
The Meso Scale Discovery R-PLEX Human IL-36gamma Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of IL-36gamma in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements This assay kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL compound and calibrator and control solutions for generating standard curves and evaluating run validity It allows sensitive reproducible quantification of IL-36gamma levels from human samples
Eurofins Discoverx PathHunter eXpress GPR146 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0365E2ACP1M)
The PathHunter GPR146 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR146 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR146 and EA-tagged β-Arrestin-2. Activation of GPR146 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Human Melanocortin 4 Receptor (MC4R) Big Sky BacMam, 15mL
Human Melanocortin 4 Receptor (MC4R) packaged in Big Sky BacMam for robust expression in mammalian cells. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.